Journal: Frontiers in Immunology

Article Title: Head and neck dermatitis is exacerbated by Malassezia furfur colonization, skin barrier disruption, and immune dysregulation

doi: 10.3389/fimmu.2023.1114321

Figure Lengend Snippet: Immunohistochemistry of HND and non-HND facial skin specimens. (A) Representative histological image at 100x magnification (left) and 200x magnification (right). Quantified staining intensity of (B) SDF-1α, (C) IL-1β, (D) TGF-β, (E) TNF-α, and (F) VEGF. (Unpaired two-tailed t-test, SDF-1α; p = 0.0014, IL-1β; p = 0.0374, TGF-β; p = 0.0058, TNF-α; p = 0.0035, VEGF; p = 0.0002) HND, head and neck dermatitis; SDF-1 α , stromal cell-derived factor-1-alpha; IL-1 β , Interleukin-1-beta; TGF- β , transforming growth factor-beta; TNF- α , tumor necrosis factor-alpha; VEGF, vascular endothelial growth factor. * : p<0.05; ** : p<0.01.

Article Snippet: Immunohistochemical staining was performed using paraffin-embedded sections with antibodies against factor VIII-related antigen (1:100, ab236284, Abcam), stromal cell-derived factor-1-alpha (SDF1-α) (1:100, ab25117, Abcam, Cambridge, United Kingdom), Interleukin-1-beta (IL-1-β) (1:100, ab2105, Abcam), tumor necrosis factor-alpha (TNF-α) (1:50, ab1793, Abcam), transforming growth factor-beta (TGF-β) (1:100, ab66043, Abcam), and vascular endothelial growth factor (VEGF) (1:200, ab1316, Abcam).

Techniques: Immunohistochemistry, Staining, Two Tailed Test, Derivative Assay

Journal: Journal of Tissue Engineering and Regenerative Medicine

Article Title: Retinal Progenitor Cells Exhibit Cadherin-Dependent Chemotaxis across Transplantable Extracellular Matrix of In Vitro Developmental and Adult Models

doi: 10.1155/2023/1381620

Figure Lengend Snippet: Summary of growth factors and extracellular matrix (ECM) used to examine the migratory behaviors of retinal progenitors cultured from Drosophila melanogaster (DPs) and from rodent (MPs).

Article Snippet: Stromal-derived growth factor 1-alpha (SDF1- α ; ThermoFisher Catalog #RP-8658) , 100 ng/mL , MPs.

Techniques: Cell Culture, Concentration Assay

Journal: Journal of Tissue Engineering and Regenerative Medicine

Article Title: Retinal Progenitor Cells Exhibit Cadherin-Dependent Chemotaxis across Transplantable Extracellular Matrix of In Vitro Developmental and Adult Models

doi: 10.1155/2023/1381620

Figure Lengend Snippet: Measurement of the chemotaxis of stem-like cells. (a) Numbers of motile Drosophila progenitors (DPs) exposed to concentration gradients of insulin ( N = 12), brain-derived neurotrophic factor (BDNF, N = 12), and pyramus (Pyr, N = 12) normalized to control (media only, N = 12). (b) Numbers of motile Mus progenitors (MPs) in response to concentration gradients of insulin-like growth factor 1 (IGF-1, N = 30), epidermal growth factor (EGF, N = 27), and stromal cell-derived factor 1-alpha (SDF-1 α , N = 28) and normalized to control (media only, N = 30). Bars indicate statistically significant increases compared to control ( ∗ p < 0.05).

Article Snippet: Stromal-derived growth factor 1-alpha (SDF1- α ; ThermoFisher Catalog #RP-8658) , 100 ng/mL , MPs.

Techniques: Chemotaxis Assay, Concentration Assay, Derivative Assay, Control

Journal: Frontiers in Immunology

Article Title: Head and neck dermatitis is exacerbated by Malassezia furfur colonization, skin barrier disruption, and immune dysregulation

doi: 10.3389/fimmu.2023.1114321

Figure Lengend Snippet: Immunohistochemistry of HND and non-HND facial skin specimens. (A) Representative histological image at 100x magnification (left) and 200x magnification (right). Quantified staining intensity of (B) SDF-1α, (C) IL-1β, (D) TGF-β, (E) TNF-α, and (F) VEGF. (Unpaired two-tailed t-test, SDF-1α; p = 0.0014, IL-1β; p = 0.0374, TGF-β; p = 0.0058, TNF-α; p = 0.0035, VEGF; p = 0.0002) HND, head and neck dermatitis; SDF-1 α , stromal cell-derived factor-1-alpha; IL-1 β , Interleukin-1-beta; TGF- β , transforming growth factor-beta; TNF- α , tumor necrosis factor-alpha; VEGF, vascular endothelial growth factor. * : p<0.05; ** : p<0.01.

Article Snippet: Immunohistochemical staining was performed using paraffin-embedded sections with antibodies against factor VIII-related antigen (1:100, ab236284, Abcam), stromal cell-derived factor-1-alpha (SDF1-α) (1:100, ab25117, Abcam, Cambridge, United Kingdom), Interleukin-1-beta (IL-1-β) (1:100, ab2105, Abcam), tumor necrosis factor-alpha (TNF-α) (1:50, ab1793, Abcam), transforming growth factor-beta (TGF-β) (1:100, ab66043, Abcam), and vascular endothelial growth factor (VEGF) (1:200, ab1316, Abcam).

Techniques: Immunohistochemistry, Staining, Two Tailed Test, Derivative Assay

Journal: Frontiers in Immunology

Article Title: Head and neck dermatitis is exacerbated by Malassezia furfur colonization, skin barrier disruption, and immune dysregulation

doi: 10.3389/fimmu.2023.1114321

Figure Lengend Snippet: In vitro experiments showing (A) increased expression levels of VEGF, VEGFR, IL-31, and IL-33 in keratinocytes cultured with M. furfur , supplemented with (B) TSLP or (C) IL-4 . M. furfur induced increased expression levels of VEGFR, TGF-β, TNF-α, and IL-1β in (D) endothelial cells supplemented with IL-4. (three replicates for each column, Unpaired t-test, (B) VEGF; p = 0.0044, VEGFR; p = 0.0019, IL-31; p = 0.0145, IL-33; p = 0.0381, (C) VEGF; p = 0.1244, VEGFR; p = 0.0244, IL-31; p =0.0382, IL-33; p = 0.0117, (D) VEGFR; p-value 0.0266, TGF-β; p-value 0.0002, TNF-α; p-value 0.0009, IL-1β; p-value 0.0009). * : p<0.05; ** : p<0.01; *** : p<0.001; ns, Not Significant (p>0.05).

Article Snippet: Immunohistochemical staining was performed using paraffin-embedded sections with antibodies against factor VIII-related antigen (1:100, ab236284, Abcam), stromal cell-derived factor-1-alpha (SDF1-α) (1:100, ab25117, Abcam, Cambridge, United Kingdom), Interleukin-1-beta (IL-1-β) (1:100, ab2105, Abcam), tumor necrosis factor-alpha (TNF-α) (1:50, ab1793, Abcam), transforming growth factor-beta (TGF-β) (1:100, ab66043, Abcam), and vascular endothelial growth factor (VEGF) (1:200, ab1316, Abcam).

Techniques: In Vitro, Expressing, Cell Culture